Division of Analytical and Physical Sciences - Department of Analytical Chemistry

Department of Analytical Chemistry

MEMBER

Research projects

　We have developed novel and “human-friendly” analytical methods in the diagnostics and treatment for diseases to contribute on the human health and welfare. Now, we are going testing in main three research projects written as follows;

１．Development of nano-clinical diagnostic methods using nanoparticles

Research1

As a theme, “How can we enhance the signal?”, we have prepared novel nanoparticles (such as liposomes and emulsions) applicable for signal generator and signal amplifier, and have further tried to develop ultrasensitive analytical methods for a variety of biomarkers in combination with their nanoparticles and luminescences (chemi-, bio- and electrochemiluminescence).

①Development of polydiacetylene liposomal aequorin bioluminescent device
②Preparation of ionic liquid nanoparticles and their application for electrochemiluminescence-immunosandwich assay

２．Development of 19F-NMR spectroscopically diagnostic methods

19F-NMR spectroscopic method can measure compounds containing fluorine atoms in biological samples without interferences caused from biological materials, because the fluorine atoms are not almost present in biological fluids.
In this study, we have synthesized novel probes detectable for cancer cells and tissues, and H2S, third physiologically active substance, and have further develop novel 19F-NMR spectroscopically diagnostic methods using their probes.

①Development of multicolor 19F-MRI probes for cancer diagnostics
②Development of 19F-NMR probes detectable for cancer cells
③Development of 19F-NMR probes detectable for H2S

３．Development of artificial immunosensor using molecularly imprinted polymer

Research3

As clinically chemical test without blood sampling and pain, we have developed ubiquitous artificial immunosensors.

①Development of potentiometrically artificial immunosensors using molecularly imprinted polymer
②Development of patch type sensors for non-invasive and easy-to-use analytical methods
③Applications of artificial immunosensors for clinically chemical test

４．Development of sensitive immunoassay based on antibody engineering

Research4

Immunoassay is a sensitive analytical method based on the antigen-antibody reactions. Its sensitivity is greatly influenced by the ability of the antibody. In this research, we will generate high-performance "artificial antibodies" using the method of "antibody engineering" in which the structures of antibodies are modified with genetic manipulation, and construct highly sensitive immunoassays.

Research Papers

2024

  1. Aki Tanaka, Atsuko Konishi, and Shigehiko Takegami: Preparation and application of multiple particle binding-liposomes for electrochemiluminescent signal amplification in bioassays. Anal. Bioanal. Chem., 416, 6451-6461 (2024).
  2. Shigehiko Takegami, Minato Danzako, and Atsuko Konishi: Detection of dopamine levels using a polydiacetylene liposomal aequorin bioluminescent device with octadecylboronic acid. Anal. Sci., 40, 353-356 (2024).

2023

  1. Yuki Kiguchi, Izumi Morita, Kouya Yamaki, Shigehiko Takegami, and Norihiro Kobayashi: Framework-directed amino-acid insertions generated over 55-fold affinity-matured antibody fragments that enabled sensitive luminescent immunoassays of cortisol. Biol. Pharm. Bull., 46, 1661-1665 (2023).

2022

  1. Shigehiko Takegami, Yuki Aramoto, and Atsuko Konishi: Spectroscopic study of cyclen-based 19F NMR probe for determination of hydrogen sulfide. Anal. Sci., 38, 813-820 (2022).
  2. Misaki Matsumoto, Hikari Sawada, Kazumi Iwata, Masakazu Ibi, Nozomi Asaoka, Masato Katsuyama, Kaori Shintani-Ishida, Hiroshi Ikegaya, Shigehiko Takegami, Atsushi Umemura, Chihiro Yabe-Nishimura: Bortezomib is an effective enhancer for chemical probe-dependent superoxide detection. Front. Med., 9, 941180, (2022).

2021

  1. Atsuko Konishi, Shigehiko Takegami: Review. Recent trends in development of electrochemical biosensors with unique sensing elements. Bulletin of Kyoto Pharmaceutical University, 2(2), 144-156, (2021).

2020

  1. Jianbo Wu, Souichi Nakashima, Marina Shigyo, Mutsumi Yamasaki, Sumire Ikuno, Aoi Morikawa, Shigehiko Takegami, Seikou Nakamura, Atsuko Konishi, Tatsuya Kitade, and Hisashi Matsuda: Antihypertensive constituents in Sanoshashinto. J. Nat. Med., 74, 421-433 (2020).
  2. Atsuko Konishi, Shigehiko Takegami and Tatsuya Kitade: Construction and Studies of Histamine Potentiometric Sensors Based on Molecularly Imprinted Polymer.Curr. Anal Chem., 16, 703-710 (2020).
  3. Shinkichi Nomura, Yoshiharu Ito, Shigehiko Takegami, Tatsuya Kitade: Development of trace analysis for alkyl methanesulfonates in the delgocitinib drug substance using GC-FID and liquid-liquid extraction with ionic liquid.Open Chemistry, 18, 1020-1029 (2020).
  4. Atsuko Konishi, Shigehiko Takegami, Yoko Idosaki, Tatsuya Kitade. Application of molecularly imprinted polymer-modified potentiometric sensor for quantitative determination of histamine in serum. Anal. Sci., 36, 1561-1563 (2020).

2019

  1. Yutaka Yoshikawa, Shigehiko Takegami, and Masateru Inoue: New approach to promoting healthy life-span with pharmacies and pharmaceutical distributors -To support the maintenance and promotion of independent healthy by local residents-. YAKUGAKU ZASSHI, 139, 511-512 (2019).
  2. Shinkichi Nomura, Yoshiharu Ito, Shigehiko Takegami, and Tatsuya Kitade: Development and validation of an assay method for benzene in the delgocitinib drug substance using conventional HPLC. Chem. Pap., 73, 673-681 (2019).
  3. Atsuko Konishi, Shigehiko Takegami, and Tatsuya Kitade: A molecularly imprinted polymer-modified potentiometric sensor for the detection of glutathione. Anal. Sci., 35, 1111-1115 (2019).
  4. Shigehiko Takegami, Atsuko Konishi, Shizuno Okazaki, Mai Fujiwara and Tatsuya Kitade: Effects of mono- and dialkylglucosides on the characterisation and blood circulation of lipid nanoemulsions. J. Microencapsulation, 36, 738-746 (2019).

2018

  1. Shigehiko Takegami, Keita Ueyama, Atsuko Konishi, Tatsuya Kitade: Combination of 1H nuclear magnetic resonance spectroscopy and principal component analysis to evaluate the lipid fluidity of flutamide-encapsulated lipid nanoemulsions. Anal. Bioanal. Chem., 410, 5033-5042 (2018).
  2. Risa Iima, Shigehiko Takegami, Atsuko Konishi, Shiori Tajima, Nao Minematsu, Tatsuya Kitade: Thermal behavior of 19F nuclear magnetic resonance signal of 19F-containing compound in lipid nano-emulsion for potential tumor diagnosis. AAPS PharmSciTech., 19, 2679-2686 (2018).
  3. Shigehiko Takegami, Kisho Watanabe, Atsuko Konishi, and Tatsuya Kitade: Formation of ionic liquid submicron particles. 1H and 19F nuclear magnetic resonance spectroscopic studies. J. Dispersion. Sci. Technol., 39, 1040-1046 (2018).
  4. Michael Mayer, Shigehiko Takegami, Michael Neumeier, Simone Rink, Axel Jacobi von Wangelin, Silja Schulte, Moritz Vollmer, Axel Georg Griesbeck, Axel Duerkop, and Antje Jutta Baeumner: Electrochemiluminescence bioassays with a water-soluble luminol derivative can outperform fluorescence assays. Angew. Chem., Int. Ed., 57, 408-411 (2018).
  5. Kazuyuki Takata, Takahide Amamiya, Hiroaki Mizoguchi, Shohei Kawanishi, Eriko Kuroda, Risa Kitamura, Aina Ito, Yuki Saito, Manami Tawa, Tomofumi Nagasawa, Haruka Okamoto, Yuko Sugino, Shigehiko Takegami, Tatsuya Kitade, Yuki Toda, William R. Kem, Yoshihisa Kitamura, Shun Shimohama, and Eishi Ashihara: Alpha7 nicotinic acetylcholine receptor-specific agonist DMXBA (GTS-21) attenuates Aβ accumulation through suppression of neutoral γ-secretase activity and promotion of microglial amyloid-β phagocytosis and ameliorates cognitive impairment in a mouse model of Alzheimer’s disease. Neurobiol. Aging, 62, 197-209 (2018).

2017

  1. Atsuko Konishi, Shigehiko Takegami, Shoko Akatani, Rie Takemoto, and Tatsuya Kitade: Potentiometric and 1H NMR spectroscopic studies of functional monomer influence on histamine-imprinted polymer-modified potentiometric sensor performance. J. Anal. Bioanal. Tech., 8, 1000378 (2017).

2016

  1. Ryoko Yamamoto, Shigehiko Takegami, Atsuko Konishi, Hikari Horikawa, Sayumi Yonezawa, and Tatsuya Kitade: Polydiacetylene liposomal aequorin bioluminescent device for detection of hydrophobic compounds. Anal. Chem., 88, 5704-5709 (2016).
  2. Shigehiko Takegami, Keisuke Kitamura, Mayuko Ohsugi, Atsuko Konishi, and Tatsuya Kitade: 19F nuclear magnetic resonance spectrometric determination of the partition coefficients of flutamide and nilutamide (antiprostate cancer drugs) in a lipid nano-emulsion and prediction of its encapsulation efficiency for the drugs. AAPS Pharm. Sci. Tech., 17, 1500-1506 (2016).

2015

  1. Shigehiko Takegami, Hidemasa Katsumi, Kei Asai, Daisuke Fujii, Taisuke Fujimoto, Haruka Kawakami, Tomomi Tokuyama, Atsuko Konishi, Akira Yamamoto, Tatsuya Kitade: Application of 19F NMR spectroscopy using a novel α-tocopherol derivative as a 19F NMR probe for a pharmacokinetic study of lipid nano-emulsions in mice, Pharm. Anal. Acta, 6, 339 (2015).
  2. Shigehiko Takegami, Keisuke Kitamura, Mayuko Ohsugi, Aya Ito, Tatsuya Kitade: Partitioning of organophosphorus pesticeides into phosphatidylcholine small unilamellar vesicles studied by second-derivative spectrophotometry, Spectrochim. Acta, Part A, 145, 198-202 (2015).